Volumetric measurement using the mammography has been done in the work which can help significantly for evaluating therapeutic response. The ability to accurately calculate the in vivo volume of tumors also has been achieved in this work. In vivo tumor volume measurement using mammography is the main strength of this study. The volume using the mammography will have been the advantage as a response evaluation tool. It can be routinely used in clinical oncology practice. Tumor volume is a more representative quantity of three dimensional tumor mass which is generally of irregular shape and size. The present study underlines the use of Doppler as its cheap examine tool for evaluation the chemotherapy response. It is another significant contribution to the existing literature. Restivity Index (RI), Pulsatility Index (PI) and peak systolic velocity are the Doppler parameters for evaluation of the drug response in cancer patients. Clinical study of the various antioxidant levels like r-SOD, r-GPx, r-catalase and FRAP observed at respective intervals after various cycles of chemotherapy and mastectomy in case of breast cancer has been done.