This project examined the use of DNA-detection technology, in particular, real-time PCR, as a means of detecting toxic cyanobacteria in freshwaters. Research and development within the project was geared toward the delivery of a technology solution for the water industry where a real need was identified. A questionnaire was developed using a literature review that included DNA-detection technologies that might be suitable for the detection of waterborne microbes. End users polled on their requirements with 63 respondents, 75% from the United States and Australia. Results are discussed concluding that real-time PCR can be used effectively to detect and track changes in populations of toxic cyanobacteria but further work on the genetics of the toxic cyanobacteria will be necessary before the quantification by real-time PCR can be used to its fullest extent.