P.C.R. 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of D.N.A. and R.N.A. in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: D.N.A. in situ P.C.R. and D.N.A. P.C.R. in situ hybridization (P.C.R. I.S.H.) for the detection of D.N.A. targets in cells; reverse trancriptase in situ P.C.R. (R.T.-P.C.R.) and R.T.-P.C.R. I.S.H. for the detection of R.N.A. targets; and P.R.I.N.S. (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.