It has been 25 years since the appearance of the now-classic Antibodies by Ed Harlow and David Lane, a "go-to" manual that has been a staple in immunology, cell culture, and molecular biology laboratories for all matters relating to antibodies. This essential book has now been revised and updated under the editorship of Edward A. Greenfield, PhD, of the Dana-Farber Cancer Institute with contributions from leaders in the field. Although some of the technology has not changed much over the years-for instance, the challenges for monoclonal antibody technology remain much the same-our understanding of the immune system and methods used to study it have. So in addition to recasting and updating chapters on the "care and feeding" of hybridomas and monoclonal antbodies with extensive new information, Antibodies, 2nd edition, presents new chapters on characterizing antibodies, antibody engineering, and flow cytometry. As in the original book, the emphasis in this second edition is on providing clear and authoritative protocols with sufficient background information and troubleshooting advice for the novice as well the experienced investigator.