This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.Contents - Symbols and abbreviations - Preface - Confocal fluorescence microscopy - Implementation - Practical limits - Digitization - Miscellaneous topics - Appendix A: Elements of optical theory - Appendix B: Formulas, relations and definitions - Appendix C: Fourier theory - Bibliography - Index