This practical book is part of the new Artech House Methods in Bioengineering series volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help you synthesize, characterize, biofunctionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by the best scientists and engineers in their respective fields, the authors offer a clear and detailed understanding of how to carry out: Nanoparticle functionalization with biomolecules, including enzymes; Nanoparticle analysis and characterization; In vitro evaluation of nanoparticles using different cell lines; In vitro evaluation of nanoparticles as therapeutics and imaging agents.