Praise for the First Edition of Protein Purification: "Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." -Trends in Analytical Chemistry. "[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." -FEBS Letters. This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: * Gel filtration and ion exchange chromatography * Affinity and covalent chromatography * Reversed phase and hydrophobic interaction chromatography * Electrophoresis in gels * Immunoelectrophoresis and capillary electrophoresis * Protein mapping by two-dimensional polyacrylamide gel electrophoresis * Protein elution and blotting methods * Large-scale electrophoresis processes.