Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology. This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition. Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research. Key Features: * Includes techniques for genome-wide expression profiling of both the pathogen and host and of the host response to infection * Cytometric analysis of cytokine secretion by immune cells * Describes tetramer technology for the quantitative analysis of antigen specific T cell responses * analysis of host cells and pathogens involved in the host-microbe interplay * Covers techniques useful for the analysis of human and murine systems * Includes techniques for the prediction and determination of MHC ligands and T cell epitopes * Covers the fundamentals and practice of DNA vaccines * Describes methods for the isolation and propagation of human dendritic cells