Malaria is infectious vector borne parasitic and Plasmodium carried by the vector mosquitoes, which belong to genus Anopheles. The first anti¬malarial developed later is chloroquine, primaquine, proquanil, pyrimethamine, metloquine etc. The aim of this study is to test the in-vitro antiplasmodial effect of extract and different fractions of Datura innoxia (leaves) and Diospyros melanoxylan (bark). D.innoxia (leaves) and D.melanoxylon. (barks), are collected from the lower forest of uttarakhand which is identified by the local people.The anti-malarial activity of the extract was discernible as number of parasite in mature schizont form in the total of 200 parasites. The crude extract (both water and ethanol) of D. innoxia and D. melanoxylon tested in-vitro had show antimalarial activity. The result of this preliminary study is very much encouraging. Natural products are important source of biologically active compounds and have potential for the development of novel antimalarial drugs....